Mouse cloned from single drop of blood
Scientists have managed for the first time to clone a donor mouse from a single blood drop collected from its tail. Although the whole process of cloning is done at a tiny level, the new study indicates that even a peripheral leucocyte collected in an hardly accessible place is sufficient to ensure a success rate of 2%. The success is part of a research program that focuses on developing effective methods of cloning.
Researchers cloned a donor mouse from a single droplet of blood taken from its tail (Wikimedia CC0)
Scientists have been working for years to develop a strain with a specific genetic mutation in mice used for research. Sometimes when specialists succeed in creating these genetically modified mice, the animals are infertile, and that’s why cloning seems to be the last option.
The genetic copies of the strains are generated by a method called somatic cell nuclear transfer (SCNT), a complex process by which an denucleated egg receives a somatic cell nucleus extracted from a donor. The use of one of the first versions of SCNT back in 1996 led to the cloning of the first mammal, the sheep Dolly.
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Cloning is currently performed using cumulus-type peripheral cells taken from a donor. These cells gather to form clusters around human follicle oocyte.
Now, the coordinators of the new study, Satoshi Kamimura and Atsuo Ogura from BioSource Center of RIKEN Tsukuba, Japan, are wondering whether also other cells easier to access, could be substituted. Samples of peripheral cells such as leukocytes have the potential to greatly reduce the risks posed to the donor during the procedure.
Research at RIKEN Center tested five different types of leukocytes. The scientists found that the highest rate of success was given by granulocytes and monocytes (cells that have a larger core).
Source: Medical Daily